We narrowed to 847 results for: PLE
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TypeBlog PostPublishedDec. 5, 2014, 2:54 p.m.... in the wired world we are living in, there are plenty of other ways to define and measure scientific ...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...
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Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....allow the extraction of enough plasmid to last for multiple experiments. Another fundamental aspect for transfection...nucleic acid and chemical contaminants, these DNA samples must also be entirely endotoxin free. Endotoxin... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m.... While those are the biggest changes, there are plenty of less obvious ones to discover as you browse.... Multiple Authors... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....experiments. Finally, we were thrilled by how many people accessed our freely available online collections...the promise of science to help a great number of people. I look forward to working with Addgene colleagues... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m.... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m.... so on, differently than scientists would. For example, “manipulate” could mean “illicit tampering” to...founder of The Addictive Brain. Prior to this, he completed PhD from Philipps-University Marburg, Germany ... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m.... never advertised. You have to meet interesting people and maintain diverse relationships to have any ...opportunities. Don't wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....metagenomic datasets to try to determine whether simpler and maybe smaller Cas systems could exist in nature... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m.... to mind! Attendees consistently mentioned how pleased they were to learn about these exciting scientific...science, all you need is a great idea and a group of people willing to make that idea a reality! How do you... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....addition of histone modifiers. “We want to allow people to prove the causal role of specific epigenetic...combine with different effectors. I’d encourage people to get creative with them for their own uses.” ... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....after vegetables; Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog...and Lukas Morgan There are many model organisms people are using for their research. In this blog post... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m....challenging – is advice and guidance from only 2 people enough? For many years I’ve been organizing mentoring...who did not serve as good mentors, but many had examples of very productive mentoring relationships with... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....arms that flank the donor plasmid cargo are complementary to either side of the double-stranded break ...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....are appying for diffrent types of positions (for example, bench research vs. project managment) your resume...a specific department and/or location. The more people you meet the better chance you have of finding ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....microbiology, virology, and genetics - before going on to complete her Masters degree at Imperial College London ...ideas with others. This love of interacting with people and talking about science makes her the perfect... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....food pantry or community arts organization. Many people are surprised to learn that Addgene is officially...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein... However, this system is far from perfect. For example, If your fusion protein is expressed at low levels...