We narrowed to 846 results for: RING;
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TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...
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Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....stain-free imaging systems are both effective for measuring the total protein present in a lane (Aldridge ...individual protein. This ensures you are truly comparing the abundance of your POI as a fraction of the... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via fluorescence changes occurring due to protein synthesis, degradation, or reconstitution... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....editing tailored to smaller patient populations. To bring DMD CRISPR therapy closer to the clinic, much work... Mary Gearing... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog PostPublishedSept. 24, 2020, 1:15 p.m....seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach.... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m.... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore... -
Help Us Name Blugene’s New Virus Friend
TypeBlog PostPublishedJan. 7, 2020, 1:55 p.m....finding a good name for Blugene’s virus friend. During the month of January, we’ll be taking name suggestions... -
2022 Depositor Week
TypeBlog PostPublishedDec. 12, 2022, 2:15 p.m....revamped Data Hub! The community needs more data-sharing heroes like you.) ... -
Introducing the Addgene Antibodies 101 eBook!
TypeBlog PostPublishedOct. 29, 2024, 1:15 p.m....to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts on an antibody-specific... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m....the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ... -
The 12 Days of CRISPR: 2021
TypeBlog PostPublishedDec. 28, 2021, 2:15 p.m....CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m.... post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also publishing research... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m.... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog PostPublishedApril 1, 2021, 1:15 p.m....navigate the scavenger hunt. Good luck and have fun exploring the blog! ... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m.... science comic not on our list? Let us know by sharing it with us in the comments. ... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....vial filling, accurate tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge...