We narrowed to 849 results for: RING;

...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...

...stain-free imaging systems are both effective for measuring the total protein present in a lane (Aldridge ...individual protein. This ensures you are truly comparing the abundance of your POI as a fraction of the...

...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159...

...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...

...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...

...editing tailored to smaller patient populations. To bring DMD CRISPR therapy closer to the clinic, much work... Mary Gearing...

...tracing techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods ...

...transferable skills while working in the lab and by offering advice on areas where you might need some help...Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional Organization Resources on...

...seems to be the case that there is no universal scoring system for selecting a gRNA, as the method of producing... is taken in the interpretation of results by requiring multiple sequences targeting a gene to score in...

...looking for, but there are also benefits gained by lowering the complexity of the screen. For example, the..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

... most common method is transfection of short interfering RNA (siRNA). Knockdown caused by siRNA is also...cytometry experiment. But validation is critical to ensuring accurate interpretation of your results. Therefore...

...finding a good name for Blugene’s virus friend.  During the month of January, we’ll be taking name suggestions...

...revamped Data Hub! The community needs more data-sharing heroes like you.)  ...

...to be a helpful desktop reference. Each chapter brings together all of our 101 blog posts on an antibody-specific...

...the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ...

...CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources...

... Although we're storing away all of our holiday decorations, our holiday spirit remains. We would like...

...navigate the scavenger hunt. Good luck and have fun exploring the blog!  ...