We narrowed to 823 results for: lars

... science and tech get jobs. She is also a very popular speaker and workshop leader. Lauren Celano of Propel...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist...

...biological questions about the brain. To address this, molecular tools to map neuronal connectivity are widely ... Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic receptors...

...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool...

...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

...browser, Ensembl, and others. It builds on existing popular web services, currently Crispor and Crispresso,...below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler. With...

...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

...up in this book. Some of these issues are not particularly new. Chapter 2, “The Professional Scientist,... process and the marginalization of science, particularly public health and environmental science, by ...

... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

...keep it holy Keep a regular schedule at work. Choose a lab/job where people have regular schedules. Work ...

...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...

...research and clinical trials, as it allows access to a larger number of willing participants. While fibroblasts...factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming...

...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because...

...undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually... iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described project...