We narrowed to 811 results for: lars

1. Negotiating Work and Life: How to Find the Joy

   Type

   Blog Post

   Published

   March 20, 2014, 3:04 p.m.

   ...keep it holy Keep a regular schedule at work. Choose a lab/job where people have regular schedules. Work ...

2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...

3. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect method... drawbacks of the competitive ELISA method are similar to those of the direct method. Namely, since the...

4. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...research and clinical trials, as it allows access to a larger number of willing participants. While fibroblasts...factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming...

5. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

6. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ... match their original description. The oldest, largest, and most diverse of these repositories is the ...out on low passage cells and this means you can regularly discard the cells you are working with because...

7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...undergo homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually... iPSC-based models of neurodegenerative and neurovascular diseases. He worked on the described project...

8. A Control for All Seasons

   Type

   Blog Post

   Published

   Oct. 5, 2023, 1:15 p.m.

   ...both groups on a western blot and look for the molecular weight shifts indicative of tau phosphorylation...against a protein standard (P) to check for tau molecular weight shifts. A negative control is a sample...

9. Transferable Skills Guide: Teamwork

   Type

   Blog Post

   Published

   Nov. 28, 2017, 2 p.m.

   ... looking at a process-oriented team serving a particular function. Project team A Project Team is assembled...an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  But some...

10. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    [email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...your plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is ...

11. CRISPR Guide Update

    Type

    Blog Post

    Published

    Oct. 10, 2024, 1:15 p.m.

    ...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

12. The 12 Days of CRISPR: 2021

    Type

    Blog Post

    Published

    Dec. 28, 2021, 2:15 p.m.

    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

13. The 12 Days of CRISPR

    Type

    Blog Post

    Published

    Jan. 3, 2023, 2:15 p.m.

    ...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th ...

14. Trends in CRISPR and SynBio Technologies

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    Blog Post

    Published

    Feb. 4, 2015, 3:58 p.m.

    ... technologies are going? What is the next hot molecular biology technology? Have you deposited your plasmids...

15. #Deckthelab Contest Winners 2017

    Type

    Blog Post

    Published

    Jan. 31, 2018, 2:44 p.m.

    ...community of creative scientists delivered some spectacular photos once again this year! All winners receive...

16. CRISPR 101 eBook...en español

    Type

    Blog Post

    Published

    Jan. 18, 2024, 2:15 p.m.

    ... 101: Una herramienta de edición  Addgene has a large international audience, including working with distributors...

17. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type

    Blog Post

    Published

    Dec. 5, 2013, 3:27 p.m.

    ...season like I do, sniffle) ourselves back into our regular post-holiday routines, why not take a little time...

18. New Videos: Addgene Lab Tips

    Type

    Blog Post

    Published

    Feb. 15, 2018, 2:41 p.m.

    ...Video Library Check out Our Protocol Pages Find Molecular Biology Resources ...

19. Twenty Years of Sharing Science!

    Type

    Blog Post

    Published

    Jan. 8, 2024, 3:25 p.m.

    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...

20. The Codon Guide to Synthetic Biology

    Type

    Blog Post

    Published

    Sept. 14, 2023, 1:15 p.m.

    ...Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions. The most useful...