We narrowed to 829 results for: lars

...inhibition, with TRIM-away targeting of mTOR producing similar results to rapamycin inhibition. To assess TRIM21... protein and the target antibody works well. In larger cell populations, you can deliver these components...

...assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With six figures....and/or apprehensive. I still get nervous quite regularly, and I've had multiple jobs where all I did was...

...Moks 1987). These tags, however, tended to be very large and often required the incorporation of a cleavage...M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like...

...increased sensitivity, polyclonal antibodies are particularly useful for certain research applications. Many...composition available. If you’re going to need a large quantity of antibody for your experiment, you should...

...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...

... science and tech get jobs. She is also a very popular speaker and workshop leader. Lauren Celano of Propel...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist...

...The raw sequencing reads are assembled into a circularized plasmid sequence de novo (without the use of...pipeline. There are regions of plasmids that are particularly difficult to sequence and assemble using the...

...biological questions about the brain. To address this, molecular tools to map neuronal connectivity are widely ... Using these technologies in combination with molecular tools (e.g., optogenetic or chemogenetic receptors...

...there’s no sense in spending valuable research dollars on a new secondary antibody if you don’t need it... better for probing poorly expressed targets. Similarly, rabbit derived antibodies may be the better choice...

...existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...Synaptophysin-GFP. Synapse targeting GCaMP6s from the Rylan Larsen lab, now available in a ready to use AAV5 viral...

...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7...

... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are now available in a ready-to-use AAV5 viral...

...browser, Ensembl, and others. It builds on existing popular web services, currently Crispor and Crispresso,...below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler. With...

... as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...ease of use, droplet digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa...

...up in this book. Some of these issues are not particularly new. Chapter 2, “The Professional Scientist,... process and the marginalization of science, particularly public health and environmental science, by ...

...keep it holy Keep a regular schedule at work. Choose a lab/job where people have regular schedules. Work ...

...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID: 26593719...