We narrowed to 713 results for: mak

1. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...physiology of different cell types, however, can make this assumption incorrect. One of the earliest steps...

2. Antibodies 101: Reading a Flow Plot

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   Blog Post

   Published

   Feb. 6, 2024, 3:13 p.m.

   ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

3. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

4. Mesothelioma - Causes, Symptoms, and Treatment

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   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma...

5. Plasmids 101: Control Plasmids

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   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

6. Plasmids 101: Biotinylation

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   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity...

8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

9. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...

10. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...allowing for stable expression of the transgene. This makes IDLVs an attractive choice for certain types of ...

11. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

12. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...position. The “sloppy” PCR reaction is created by making some or all of the following modifications: 1) ...

14. Evolution of Lab Techniques

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    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic mouse lines...

15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...model than in latent HIV-1 infection, which would make it easier for an “escape” virus to propagate and...

16. CRISPR 101: Homology Directed Repair

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    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...register at a time. If a particular application makes it difficult to isolate single registers or if it...

18. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ... high degree of variability in the process could make it difficult to obtain meaningful results.  Biological...

19. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...overhangs that allow them to bind. Ligase is used to make bonds between the insert and backbone covalent.  ...

20. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...Addgene’s Website and travel to meet scientists, making my job different every day. I love the freedom ...