We narrowed to 629 results for: Dos;

...components can interfere with conjugates. Make sure to do your research before conjugating at the bench.   ...to distinct, non-overlapping epitopes so that they do not interfere with each other. The detection antibody...

...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018... identifying strong hits in genetic screens, they do have several limitations. CRISPRko results in a complete...

...fluoresce, the Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro...recognition of ions or particularly small molecules that do not generate an immune response. Aptamers can be ...

...negative control to determine whether your PIPs actually do interact. Choosing the right negative control is ...scenarios that call for a simple, yes-no answer — i.e., do two proteins interact or not? — FRET is preferable...endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This is straightforward in easy-to-transfect...easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also theoretically...

...but orthologs like LwaCas13a do not require a specific PFS. Cas13 enzymes do not contain the RuvC and HNH...

...cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular biologists working with ...in order to understand what these vector particles do once they come in contact with target cells (Fig....

...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...

...time, when scientists who support open science, but do not share their own data - which seems to be the ...members or colleagues: How much unpublished AAV data do you have sitting in computers and notebooks right... gene transfer to the rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy...

...offer tools to help with these decisions.  So when do you want to codon optimize your gene of interest?...useful in expressing functional proteins in hosts that do not naturally express that gene. The protein you ...