We narrowed to 821 results for: LAS

...2011). But this requires other lasers than the standard Ti:Sapphire laser. It is also important to finely...Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic transition...two-photon probes when using a standard Ti:Sapphire Laser include: Blue-green fluorescent proteins like EGFP...fluorescent proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with ...researcher can flank fluorescent proteins in a plasmid with loxP sites such that their expression is turned... the brains of mice chronically infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009...cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog 5.7 (2009): e1000505. PubMed...

...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...cell membranes, so they are best used either with plasma membrane proteins (such as GPCRs, ion channels,...40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized cells  Cy5NTAc...38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized cells  Cy5NTAc...

...transformation Read our Plasmids 101 blog posts Resources on Addgene.org Browse our Plant Plasmids and Resources...page Find synthetic biology plant plasmids Find CRISPR plant plasmids ... the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated in the medicinal...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ...its function. In nature Agrobacterium contains a plasmid with a T-DNA (transfer-DNA) region. During infection...localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different leaf...

...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...computer. Eventually, our workflow would handle plasmid sequences on a 96-well plate basis, but for rapid...

...suppressed and NES activity is enhanced, leading to cytoplasmic translocation of the reporter. As a result, the...phosphorylation status. The relative ratio of cytoplasmic vs. nuclear fluorescence serves as a proxy for...monitor global kinase activity that occurs in the cytoplasm and/or nucleus, while FRET-based reporters can...because KTRs shuttle between the nucleus and the cytoplasm, nuclear export and import rates can influence...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...

... disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the...the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease ...in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with other genes... their function, and C. elegans overexpression plasmids to upregulate their expression in specific cells...tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown, or overexpress... Support Rare Disease Research: IFOPA Donating Plasmids to Researchers in Developing Countries with Seeding...Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile... These two steps are (Figure 1): The initial plasmid integration step (the so-called “single crossover... an allelic exchange experiment? Find the pTOX plasmids here! Continued development of new allelic exchange...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn...recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our molecular biology...

... eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection pages... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...calcium signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...pages Read our molecular biology reference Find plasmids for your research ...

...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...18454154. PubMed Central PMCID: PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create functional...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of the 16 non-visual...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists...drugs to further understand their mechanism. These plasmids are available as a collection, facilitating screening...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...