Skip to main content
Addgene
Showing: 351 - 360 of 598 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro ...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...would be useful for their own research, and helps requestors use the plasmid in their own labs. We highly ...survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such as some...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...236.
 Pubmed. P.A Santi. (2011). Journal of Histochemistry & Cytochemistry, 59, 129-138. Pubmed. J. Huisken...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've combined our...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ..., M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression from a replicating...PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. J., & Wold, W....

  6. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...some integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org... Tissue Fixation & the Nature of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com...

  8. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...to late-career grant professionals have similar stories about a time when their passion for an organization...their new career path.  A part of the career path story for most established grant professionals is learning...

  9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...including MTA and shipping. Addgene realizes the One-Stop shopping for a wide range of plasmids. The merit... Their progress might be slow, but if they don't stop, they may be able to achieve something that may ...

  10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...
Showing: 351 - 360 of 598 results