We narrowed to 884 results for: ica

... as a helicase. Following recruitment by Cascade, Cas3 nicks the non-target strand. The helicase domain... Cas effectors to carry out genomic editing. Specifically, these types of CRISPR systems work using a ...recruit Cas3, which carries out the brunt of the mechanical work for editing. Class 1 CRISPR systems make...After these initial steps, Cas3 can perform its helicase and nuclease functions simultaneously, by continuously...debilitating disease! This is, of course, only hypothetical at this point. More time and energy must be ...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...unidirectional genome editing in human cells. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-...

...CRISPR screens check out Addgene’s 2016 publication “Practical Considerations for Using Pooled Lentiviral...experiments you use them for! A well-developed biological question and experimental system are absolutely...coli, and T. gondii genes.  2) What genetic modification are you trying to make? Addgene carries CRISPR...next-generation sequencing to check the quality of your amplification. If you've obtained a ready-to-use lentiviral... As such, sequencing CRISPR libraries after amplification and after a screen requires the use of next-... to skewed results. Selecting a cell type - Theoretically, any cell type can be used in a CRISPR screen...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

...with the newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR...it's usually more efficient than plasmid donor. Typical design is to have 50–80 bp of homology arms on ...changes (>100 bp insertions or deletions), we typically use plasmid donor, with two homology arms on each...transfection in cells like HEK, we use around 400 ng of a typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector...plasmids. For ssDNA oligo design, the Zhang lab typically uses around 100–150 bp total homology. The mutation... giving 50–75 bp homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes...100 bp insertions or deletions), the Zhang lab typically uses a plasmid donor, with two homology arms on...

...When Jessica Sacher, a microbiologist from the University of Alberta, saw that scientists were using ... request for phages to the directory does not typically come out until nearly the last minute when all...all other options have been exhausted. Theoretically, phage alerts can be sent out to the directory earlier...team must decide which labs should receive the clinical sample to test their phages. Because the sample...phages targeting organisms closely related to the clinical sample. For treatment, it is best to identify ...researcher may only know how to prepare phage for one application but not another or they may have only used phage...phage in basic research but not therapeutically: not all labs in Phage Directory have the resources or...

...Regulatory DNA function is typically inferred based on correlative biochemical characteristics and ectopic...Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint... of Visualized Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details... size and frequency [3]. There are several applications in which the production of deletions by paired...useful to study haploinsufficiency. Subsequent modifications at the remaining allele within the deletion ...for deletion screening by conventional PCR. Biallelically disrupted cells can be easily identified. Even...investigator at Boston Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics ...

...my opinion, it was more important to communicate the applications of CRISPR over the details of how it...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...parents who can answer some of the surprisingly technical questions elementary students can ask. It doesn...unlike elementary schools, middle schools have dedicated science teachers. I tried contacting our school...simplistic and I would insult the students. Too complicated and I would quickly lose their interest. I won...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on... dangers of people using the technology in an unethical manner, but I also tried not to be an alarmist...

...fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...of Fixatives in Histology. Leica Biosystems. https://www.leicabiosystems.com/us/knowledge-pathway/fixation-and-fixatives...-linking cellular proteins. Cross-linking is a chemical reaction that covalently joins two molecules and...proteins can be lost during fixation with these chemicals, which impacts sample structure and could also... target to be washed away. Furthermore, these chemicals can also alter the structure of proteins, which...Tween-20, or saponin. Each detergent has different chemical properties that will impact the level of permeabilization... don’t even involve antibodies, they are still critical to the outcome of your IF experiment and deserve...

...viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific...of these discussions as a publicly accessible practical guide which was recently published in Frontiers...of molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT...transgene expression by restricting expression to genetically defined cell types. Specific transgene expression...defined set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific...result in opsin expression in their cell bodies. Optical stimulation in a different brain area can then ... more versatile. To complement the wealth of genetically encoded tools available, researchers are also...

...scientists are overrepresented on Twitter Mathematical, life, and physical scientists are underrepresented. It...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...from the United States Standard Occupational Classification System  and Wikipedia. 2. Social and computer...Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific Domains Tweeted by Biologists...shown. Top: The follower network of life (green), physical (blue) and social (orange)  scientists largely...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...

...for both transient and stable gene expression applications in plants. This smaller, easily customizable...Agrobacterium are termed binary vectors because they must replicate in two species: Agrobacterium and E. coli. Like.... coli. However, they also need to be able to replicate in Agrobacterium to ensure that they are maintained... Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium, along...bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they...constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized in studies...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...