We narrowed to 851 results for: CAL

...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out. Conclusions...

... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...arrays, they did not know their functions and simply called the repeated elements “direct repeats” and the ...

...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG...

...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

... specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused...previously uneditable sequences. Four DNA base swaps, so called point mutations that change C to T, G to A, A to...

... a downregulation of signal, though it’s hard to call from this plot. The red (bottom) peak overlaps with...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...

...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...

...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability... and “Sequence” tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website...

... the following, which are later printed for my physical lab notebook, along with any figures generated...between each word can make it difficult to accurately call a file. Version control your commonly used scripts...

...intuitive, or logical to a biologist that are not at all obvious, intuitive, or logical to a layperson...

...out the sources of miscommunication and b) find practical solutions.  My PhD prepared me remarkably well...answer to these questions--which were real, not hypothetical--was clearly “no.” When there was room for misinterpretation...

...once it is engaged by its target, it will non-specifically cut ssDNA. This feature is shared by other Cas... potential as a viral therapeutic that could specifically degrade target viral RNA. For more information...

...library, propagated it, separated it into roughly identical replicate groups, and treated these groups with...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment...

...Another strength of Golden Gate cloning is its scalability. Unique 4 base overhangs can be used to assemble...design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences that...

... will provide an initial consultation meeting or call for free.  Take advantage of this to get the information...blogged for us) offers a free report on her website called “Secrets to Success in Graduate School”. She has...

...given the data provided by the depositing lab, we call these “quality control (QC) issues.” We then ask..., Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation...

...to visualize DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

... Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel recently took a different ...Strategy #2 (ProB): Create promoters using phylogenetically conserved sequences that are upstream of the...

...search algorithm rather than pre-designed sets, so identical searches can return multiple answers. You might...might want to take advantage of their save and recall functions when you get results you like. NEBridge ...