We narrowed to 771 results for: INA
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TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....precisions pipetting, and good record keeping. Finally, if you are a fellow researcher interested in adapting...
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Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m.... variants have two endonuclease domains: the n-terminal RuvC-like nuclease domain and the HNH-like nuclease... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....similarly to his advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... finding and joining your dream lab, watch the webinar "How to Choose Your Next Lab" (updated 2020 version... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m.... Overview of AAV Encoded Calcium Biosensors Illuminating Epigenetics with a FRET Based Biosensor Fluorescent... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....is not currently known to cause any diseases. Recombinant AAV largely does not integrate, and the addition... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m.... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to engineer... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m....use BioBuilder as an afterschool program or as a final project. BioBuilder’s open-access curriculum begins... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m.... number of target DNA molecules present in the original sample with a great deal of precision. This means... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....Note: this plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....synaptic transmission. Cleavage of VAMP2 renders it inactive, thus inhibiting neurotransmitter release. How... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....be detected by our in-house software and also eliminates many of the extraneous ORFs that cluttered our... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your choice... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m..... (To be completely accurate, IPTG binds and inactivates the lac operon repressor, thereby allowing lac... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m.... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....energy and effort into making the startup self-sustainable. Within a year from this decision, Mike and his... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....feedback for improvement. Go to a science or career seminar together and meet to debrief on the topic (career...