We narrowed to 729 results for: NSI;
-
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....inverse Orientation), FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts...
-
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....it’s too inefficient and the reagents are too expensive to make it practical - but we have members looking... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m..... They will make mistakes and you will take responsibility (within reason!). Try to buffer them from ... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....deposition of the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m.... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m.... from this data is that CRISPR technology is consistently showing rapid development and quick adoption... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....think plant biology is massively underfunded considering how important it is. I would really like to reach... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial genomes with... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources... -
Transferable Skills Guide: Time Management
TypeBlog PostPublishedDec. 5, 2017, 1:52 p.m..... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...