We narrowed to 751 results for: Nes;
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TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....exceptions to our standard workflow. Table 1: Guidelines for the data fields in the deposit spreadsheet...
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A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....maintaining the quality of reagents, especially shared ones. The importance of QC Plasmids are a fundamental... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m.... 500 times the number of plaques than the linear ones. The 52-piece circular assembly of the T7 genome... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....S. (2019). DREADDs: The Power of the Lock, the Weakness of the Key. Favoring the Pursuit of Specific Conditions... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....something that made them better than my unsuccessful ones. With grant applications there’s going to be an ... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m.... Abcam and NEB have Ph.D.s answering emails and phones). We can’t be experts on every technique or experiment... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....orientation, and type of target sites that flox your genes of interest, a powerful FLEx switch can be created... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software produces quantifiable... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....best performer mGold — while maintaining high brightness and fast maturation time (Figure 1). See the ... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems for genome engineering... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room for improvement... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....interview or campus visit in person, things like sickness or scheduling issues can and do interfere with... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....BioMolecular Modeling, we spun off a small, family-run business – 3D Molecular Designs – to commercialize some... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....science outreach: engaging the public to promote an awareness and understanding of science. Like most science... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...