We narrowed to 787 results for: POR

1. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...you to systematically study gene function with temporal control and cell-type specificity! The Gal4/...

3. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

4. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

6. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

7. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

8. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

9. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

10. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

11. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

12. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

13. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

15. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

16. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

17. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

18. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

20. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...