We narrowed to 783 results for: POR

1. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

2. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

5. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

7. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

8. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

9. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

11. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

12. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

13. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

14. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

15. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

16. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

18. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...