We narrowed to 792 results for: Por

1. Visualizing Genomic Loci with CRISPR-Sirius

   Type

   Blog Post

   Published

   April 18, 2019, 12:53 p.m.

   ...robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements...

2. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...

3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

   Type

   Blog Post

   Published

   July 21, 2020, 1:15 p.m.

   ...equipment, and expected DNA concentration. It’s important to consider the advantages and disadvantages of...

4. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...session you’re presenting is eye contact. “It’s important to keep your video on and look into the camera...

5. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

6. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

7. DAP arrays for multiplex gene editing

   Type

   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

8. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

10. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

12. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

14. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

15. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

16. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

17. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

18. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

19. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

20. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...