We narrowed to 781 results for: Por

1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

3. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

4. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

5. How to Keep a Lab Notebook for Bioinformatic Analyses

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   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

7. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

8. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

9. Antibodies 101: Flow Cytometry Controls

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   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

10. Viral Vectors 101: Biosensors

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    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

11. Viral Vectors 101: Types of viruses

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    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

12. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

13. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

14. Antibodies 101: ChIP

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    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

15. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

16. Plasmids 101: Blue-white Screening

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    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

18. CRISPRainbow and Genome Visualization

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    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

20. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...