We narrowed to 855 results for: REN
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TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....interests, and spends one-on-one time with the current members to share short-term goals and chat about..., but even brief stays there can make all the difference. Choose a lab that brings on the happy dance ...
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Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde serotype...significantly updated in 2023 by Jason Nasse. References 1. Tervo, D. Gowanlock R., et al. "A designer... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....optimized for each specific sample type. And, if that weren’t bad enough, optimization of one step can affect...protocols when embarking on your ChIP journey! References and Resources More resources on Addgene’s blog... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....illustrates the utility these tools have in many different areas of biology as they grow. The top 15 plasmids...envelope protein enables this virus to infect many different cell types so it is useful in a wide range of ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene...static image file (PNG, see above) for your own reference or to paste directly into your notebook. This ... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....peptide. By combining three different localization peptides with four different fluorescent proteins, and... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....contain 3 methylases that recognize and methylate different stretches of DNA: Dam methylase adds a methyl...could overlap with a methylation site. The quick-reference table below lists 10 common enzymes that may be... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....your working directory (the folder that you are currently working in) every time you use them, leave them...guest blogger Kate Palozola! Kate Palozola is currently a graduate student at University of Pennsylvania... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....organizations, knocking on doors, and attending conferences. And when CRISPR arrived in 2012, our repository...’s booth at the Society for Neuroscience 2023 conference. In 2020, the world changed in response to the... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....probably aware that there are several methodologies currently available for approaching it. These include the...even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....transfer, each GOI is usually expressed from a different plasmid, which may lead to unequal gene dosage...consists of 4 plasmid toolkits generated with 4 different, binary 2in1 vectors with all possible combinations... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....and Gammaproteobacteria species isolated from different bee species. BTK can be used to introduce fluorescent...distributed cell division counting plasmids here References Leonard, Sean P., et al. "Genetic engineering... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....fail you. The benefits of lab management software aren’t just limited to large volume facilities; it can...above, any one of these steps can fail but, in our current setup, there are no explicit instructions on what... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m.... such that they activate gene expression from different promoters. Moving to an in vivo system, Tang et...eager to see how you use them in your research! References 1.Tang, Jonathan C.Y., et al. “A nanobody-based... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques? Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... health and safety department if they are not currently provided. Eye protection or face shields are ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....-Wagner’s lab produce different levels of dCas9 expression and contain different antibiotic resistance... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....more precise editing in the lab. References and resources References Dong D, Guo M, Wang S, Zhu Y, Wang...