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We narrowed to 879 results for: REN

Showing: 701 - 720 of 879 results
  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...
  2. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...
  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...
  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...
  5. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad Institute...Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University  University...
  6. Behind the Scenes of Addgene’s New Look

    Type
    Blog Post
    Published
    April 28, 2026, 1:15 p.m.
    ...chosen to help convey our personality — Addgenies aren’t afraid to have a little fun! Figure 2: ...our evolution, it then turns to show Addgene’s current headquarters, where we moved in 2019 as we grew...
  7. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...
  8. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...
  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...more efficient and feasible for many labs.  References Efficient Intracellular Delivery of Native ...into all cells? Schwarze SR, Hruska KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22...
  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...
  11. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...provides something that you can’t obtain at your current institute. A common reason is hands-on training... right for you, start thinking outside of your current research box, send out some feelers, apply to programs...
  12. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...
  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...multiple samples without cross contamination. We currently use the CosMCPrep system for this, but thought... because it forced workshop participants from different biotech companies to interact and learn more about...
  14. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...have made it easier to track translation in two different ways: by monitoring the first round of translation...various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide ...
  15. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...
  16. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP Monoclonal...
Showing: 701 - 720 of 879 results