Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 848 results for: STI;

Showing: 701 - 720 of 848 results

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...sequences with G/C content outside this range can still be optimized (Anzalone et al., 2019). Test different... edit. If possible, consider following these suggestions: Edit the PAM along with your intended edit....

  4. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...sample protein is retained better, antibodies will stick permanently to a membrane that has been dried, no...harsher stripping means more sample loss. If you are still optimizing your system, start with a mild stripping...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...modifying enzyme) to pair with it can be queried by testing any number in the Level 2 assembly. These pathways...scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, synthetic ...

  7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...proteins, it’s important to note that despite their distinction by name, chromoproteins are actually a subset...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

  10. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...limited to restriction enzymes - although many labs still love using them! With so many technologies currently...an ordered set of scarless ligations following digestion with Type II restriction enzymes. All of these...

  11. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

  12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements in order...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...

  15. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...

  16. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation, the original...and XhoI MluI BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional...

  17. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...viral vector production facility, food allergy testing lab, and the grad student down the hall from you...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...studies), as well as translational applications (mechanistic studies and gene therapy treatments for neurological...

  19. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...PMCID: PMC5915492. Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet...

  20. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells... for cancer immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers...
Showing: 701 - 720 of 848 results