Addgene

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Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

Type

Blog Post

Published

May 25, 2021, 2:29 p.m.

...reagents and will enable researchers everywhere.” The timing is ideal as the NIH’s BRAIN Initiative has been...
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

Type

Blog Post

Published

April 23, 2020, 1:15 p.m.

...eliminates the double-stranding step and reduces both the time and cost for AAV genome sequencing. Figure...
Celebrate Open Data Day with Addgene’s AAV Data Hub!

Type

Blog Post

Published

March 5, 2020, 2:15 p.m.

...titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection did you wait...
New and Upcoming Viral Vectors - December 2019

Type

Blog Post

Published

Dec. 19, 2019, 2:40 p.m.

...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...
Antibodies 101: Epitope Availability

Type

Blog Post

Published

Jan. 11, 2022, 2 p.m.

...antibodies, which only recognize one epitope. (It can sometimes be an issue with polyclonal antibodies, so it'...
Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Type

Blog Post

Published

July 7, 2015, 3:36 p.m.

...requires less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging...
Another Pathway into Cells: iTOP

Type

Blog Post

Published

June 23, 2015, 8:37 p.m.

...Compared to CCP transduction, iTOP is at least four times more efficient in primary fibroblasts. iTOP also...
Viral Vectors 101: Inverted Terminal Repeats

Type

Blog Post

Published

Oct. 19, 2023, 1:15 p.m.

...not properly assemble, but these sequences can sometimes be removed to improve vector stability and sequencing...
Hot Plasmids - January 2023

Type

Blog Post

Published

Jan. 31, 2023, 2:15 p.m.

...of our most commonly requested antibodies. But sometimes, one antibody per target just isn’t enough and...
Pushing the Limits of DNA Assembly

Type

Blog Post

Published

Sept. 6, 2017, 1:01 p.m.

...transformation can take 5 days. NEBuilder shortens that time to one day by simplifying the protocol to a single...
R Bodies: Membrane-Rupturing Microscopic Tools

Type

Blog Post

Published

April 14, 2016, 2:30 p.m.

...then amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical...
Creating accessible biology activities in schools with BioBits

Type

Blog Post

Published

Aug. 2, 2018, 12:56 p.m.

...teaching developmental biology Use the pathways over time plasmids to engage students in functional genomics...
FPbase: A new community-editable fluorescent protein database

Type

Blog Post

Published

May 16, 2018, 1 p.m.

...these tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current ...
The Importance of High Titer for AAV Transductions

Type

Blog Post

Published

March 1, 2018, 2:11 p.m.

...Nonetheless, there are a variety of ways you can optimize AAV production. We’ve listed some simple steps...
New and Upcoming Viral Vectors - June 2019

Type

Blog Post

Published

June 18, 2019, 1:15 p.m.

...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...
Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

Published

March 14, 2019, 1:54 p.m.

...PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of Exosome-Associated...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

Published

Aug. 2, 2022, 1:15 p.m.

...more specific procedural instructions, including timing, preparing the smear, and mixing the stains and...
Using CRISPR/Cas9 to Edit Disease Out of the Genome

Type

Blog Post

Published

June 23, 2022, 1:15 p.m.

...scientists are using sgRNA for selection and optimization. In addition, the current work in developing...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

Published

Sept. 28, 2016, 2:35 p.m.

...deeper into the world of stem cell scientists. In my time at the meeting, I've made some observations regarding...
Resume Writing for Non-academic Science Careers

Type

Blog Post

Published

Dec. 18, 2014, 4:06 p.m.

...'t think a resume is "one size fits all" like sometimes CVs are. Because you only have two pages, you ...

Showing: 701 - 720 of 797 results

Results per page:

Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

Celebrate Open Data Day with Addgene’s AAV Data Hub!

New and Upcoming Viral Vectors - December 2019

Antibodies 101: Epitope Availability

Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Another Pathway into Cells: iTOP

Viral Vectors 101: Inverted Terminal Repeats

Hot Plasmids - January 2023

Pushing the Limits of DNA Assembly

R Bodies: Membrane-Rupturing Microscopic Tools

Creating accessible biology activities in schools with BioBits

FPbase: A new community-editable fluorescent protein database

The Importance of High Titer for AAV Transductions

New and Upcoming Viral Vectors - June 2019

Improving Transduction Efficiency with Exosome AAVs

Plasmids 101: Gram Negative and Positive Bacteria

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Four Factors that Differentiate the Stem Cell Field

Resume Writing for Non-academic Science Careers

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