We narrowed to 745 results for: TRI

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those...

2. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...with the desired fluorophores. In figure 4B below, triple colocalization is possible even when staining cells...

3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia ...

4. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...these systems in your research? Cary Valley contributed to updating this article. References Cox, David...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...immediately after it has been heat-shocked or electrically zapped. As a result, a shorter recovery period...

6. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   Published

   Feb. 9, 2023, 2:15 p.m.

   ... students work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school...

7. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   Published

   Feb. 6, 2020, 3 p.m.

   ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...

8. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ...viral genome (Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must ...

9. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving CRISPR...

10. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    .... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently...

11. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length...

12. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...vivo assays in which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far...

13. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures...

14. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...and Frasch, 1993).  Tinman, which is spatially restricted to the dorsal mesoderm, is necessary to activate...

16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    .... APOBEC-Cas9n-UGI targets its preferred trinucleotide motifs (5’-TCA-to-TUA) found within the 43 base-pair...

17. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...that this system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It...

18. Hot Plasmids: Fall 2024

    Type

    Blog Post

    Published

    Nov. 21, 2024, 1:30 p.m.

    ... & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency...

19. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...of flow cytometry is to use increasingly more stringent markers to identify your specific cell population...

20. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ...However, a key limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate...