We narrowed to 738 results for: TRI
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TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...
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Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....viral genome (Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must ... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....Beisel et al., licensed under a Creative Commons Attribution 4.0 International License. Improving CRISPR... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m..... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....Unlike other epitope tags – which when doubled or tripled increase the tag size quickly – modifying the length... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....However, a key limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....vivo assays in which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m.... cognate tRNA abundance Repetitive sequences Restriction sites Sequences prone to create secondary structures... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m.... & Randall, R. E. (1992). Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....and Frasch, 1993). Tinman, which is spatially restricted to the dorsal mesoderm, is necessary to activate... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m..... APOBEC-Cas9n-UGI targets its preferred trinucleotide motifs (5’-TCA-to-TUA) found within the 43 base-pair... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....that this system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....of flow cytometry is to use increasingly more stringent markers to identify your specific cell population... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m.... This post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....selection of proper experimental controls is not a trivial endeavor, as biological systems have many variables... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....researchers at the NIH here. Note: A. Max Juchheim contributed to the writing of this article. Additional Resources... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....This post was contributed by guest bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....Waldman, Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs...