We narrowed to 852 results for: ell
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TypeBlog PostPublishedJune 17, 2015, 9:04 p.m....written content about timely scientific topics, as well as archived seminar presentations. One of SITN'... In return, the public receives trustworthy, intelligible scientific resources. SITN hopes to make DayCon...
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Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog PostPublishedAug. 13, 2020, 1:21 p.m.... an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role in the company. One ...Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke with amazing people that were... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m.... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed. A cell-type specific Cre can provide you with that control... -
What's New in CRISPR - December 2019
TypeBlog PostPublishedDec. 17, 2019, 2:37 p.m....mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m....pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....upload and share their negative data. After all, they tell me, when something doesn’t work, it’s not a failure...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m.... you cite it using both its Addgene ID number as well as the reference for its original publication. To...expressing different types of rhodopsins and sensors as well as references and links to other resources. If ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m...., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....like the cartoon on the right?!? I would love to tell such a dramatic story, but it wouldn’t be my story...career while still in graduate school, you’ll be well suited to follow your passion and land a position... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....isolated resorts creates a sense of camaraderie with fellow attendees from the moment you arrive. Getting off...alone were worth the trip. Dr. Dana Carroll's excellent keynote address was the first of 19 talks given... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m.... can be used to visualize cells and to quantify targeting of particular cell types. For example, the mDlx... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m.... or more “important”, they just need to know you well enough to ask good questions and hold you accountable... can share it with others and get their input as well. Giving a great video presentation is going to be... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....address this, several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS)... small amount of spurious DNA from the packaging cell line, helper and packaging plasmids. We decided ... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m.... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction...fantasy, pop-sci, true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice...