We narrowed to 910 results for: ide

... founding Addgene, where the idea for Addgene came from and how the idea got off the ground Chapter 3 ...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

... represents the continuation of our efforts to provide resources to researchers in developing countries...Ghanaian women by including PCR screening for HR HPV alongside the cytological tests currently in use. Gladys...screens. She hopes that these screens will both identify individuals who are infected with HR HPV and therefore...

...Studies Hybridoma Bank. Antibodies are the most widely used class of protein-binding reagents. In the ...and expanded. The clonal cells are screened to identify hybridoma lines, each producing a monoclonal antibody...ensuring affordable access to materials, repositories provide standardized quality-control and technical support...

...-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said that the new system could...likely to work in animal embryos based on earlier evidence showing that a CRISPR/Cas9 plasmid could be injected...vector-mediated multiplex genome engineering. The system now provides an efficient targeting strategy for multiplex ...

...citation. If you’re considering requesting an Addgene antibody, the Data Hub can help you decide if an antibody... your application. It can be quite difficult to decide on which antibody to request for your experiment...

...student in the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria...bacteria) for use as antibiotics. To develop this idea, Drs. Koeris and Lu founded the biotech startup ... as antibiotics 8:26 - 10:30 : How Dr. Koeris' decided to develop phage for use in a biotech startup 12...

...visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies represent...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from Our Bloggers...

...the DNA sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and... might suspect contamination in your sample or consider asking your sequencing facility to apply a special...vendors. The precipitation method has an unfortunate side effect of messing up the reaction around base 70...

...Stanford University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc...acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows...Microbial World’ showcased complex connections and ideas in various visualized approaches from hand-drawn...

...committee creates a compensation philosophy that helps guide employee compensation and also serves as an objective...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

...on the Addgene blog and have created and updated guides on various topics in biology over the past year...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and...

...Pooran surveyed a CRISPR google forum to get a better idea of how many people are having trouble, to determine...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing Lab...

...high-throughput manner, but these systems have not been widely employed in vivo. The CHIME and new X-CHIME systems...deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene knockout in immune systems...CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in Mammalian...

...treatment, it is best to identify a cocktail of 3-12 phages. Once these phages are identified, they must then ... forward to help, the Phage Directory team must decide which labs should receive the clinical sample to...

...may have only 2-3 formal supervisors that will provide guidance and experience. Navigating a fulfilling...about mentoring In this blog series, I plan to provide answers to the top questions scientists ask me ...teaches you to fish, doesn’t fish for you) Helps identify a Mentee’s strengths and make the most of them...

... Industry webpage is a great starting place to identify materials that may be useful to your research....construct can be used with the AI generated synthetic guide RNA sequences found in OpenCRISPR-1 sgRNA-16nt stem...researchers to visualize single molecules and nascent peptides in live cells. These scFv hybrids have specificity...

...Protein Collection webpage. These plasmids are divided into the following categories: Over 300 empty ...localizes in the cell, use these markers as your guide. As Michael Davidson told the Tallahassee Democrat...go together, we can fix it. Right now, we have no idea." Now, it will be that much easier for scientists...

... generosity makes it possible for researchers worldwide to access the tools they need, accelerating scientific...educational resources, you're an essential part of a worldwide community helping scientists access the tools ...part of ours. We’re grateful to move into 2026 alongside you, strengthening the foundations of science ...

...viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure... deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database...molecular biology protocols Browse our protocol video collection ...

...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally...