We narrowed to 792 results for: por

1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...session you’re presenting is eye contact. “It’s important to keep your video on and look into the camera...

2. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   Published

   Aug. 2, 2022, 1:15 p.m.

   ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

3. DAP arrays for multiplex gene editing

   Type

   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

5. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...you to systematically study gene function with temporal control and cell-type specificity! The Gal4/...

6. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

7. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

8. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   Published

   Oct. 9, 2014, 3 p.m.

   ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

10. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    Published

    Aug. 20, 2024, 1:15 p.m.

    ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

12. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

14. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

15. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

16. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

17. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

18. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

20. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...