We narrowed to 629 results for: Dos;

...gradients, practice making the iodixanol layers before doing your first purification. This helped me get a feel...to learn more about different ways to titer AAV. Do you have any AAV production tips or tricks? Please...multiplicity of infections (MOIs), to determine the optimal dose of AAV for my cells of interest. Every batch of ...

...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

...of necessary data requirements and likely will not do so (calling it a “sisyphean task” given the endless...Firstly, we rely on many other labs to teach us how to do experiments and understand their data. Likewise, ...

...quantification so that you can focus on what you want to do with your plasmid without spending time and resources...

... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

...other aspects of molecular biology. “What I'd like to do with the plasmids from Addgene is have the students...

...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...