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We narrowed to 816 results for: LAS

Showing: 361 - 370 of 816 results

  1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ... of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria links the...encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection...Phage-assisted evolution of base-editing activity. A plasmid-based genetic circuit in the bacteria links the...off-target RNA editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors...editors for your research Read our CRISPR guide Find plasmids for your research ...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...have pre-designed plasmids for these systems! Ion sensors One common biosensor class is designed to monitor... involve fluorescence, pH change, heat, etc. The last component of the biosensor is the signal transducer...example, calcium dynamics in the cytosol and sarcoplasmic reticulum are of interest to muscle biologists...factors Unlike the previously described sensors, this class measures either factors as proxies for a cellular...specific sensors designed for them. For example, this class includes tension monitors associated with specific... vehicle, we’ve got you covered with packaging plasmids! References and Resources References  Bhalla ...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids in the...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral... virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on donations. That seed...depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently sharing viral preps) through...positive response to the option of Addgene providing plasmids in a ready-to-use viral format. Having identified.... With their expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping...

  4. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...imaging with newer lasers in the range of 1000–1080 nm. These industrial fiber lasers are becoming increasingly...different color of light and be compatible with the lasers available in your microscope setup. Figure...excitation wavelength. OCaMP is well-suited for these lasers, with two-photon excitation at 1030 nm, enabling...imaging, there's a version that fits your setup: Plasmid Backbone Promoter pRSET OCaMP pRSET ...button_text" is_json="true" %}{% raw %}"Find OCaMP plasmids available at Addgene!"{% endraw %}{% end_module_attribute...on addgene.org Fluorescent Proteins: Biosensor Plasmids In-Stock AAV Vectors: Biosensors AAV Packaged ...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...down into 5 key steps: Construction of the rAdV plasmid (~1 week): The AdEasyTM system is the most popular...adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest...how to generate the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide...2.*Pro-Tip*: once the correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction...-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and allowed to stay in culture...transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...

  7. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche... "Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell (2015). PubMed PMID: ...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche .... Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092....cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova...Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems. 5. Nat Rev Microbiol...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...AAV vectors is the Allen Mouse Brain Connectivity Atlas, a project to map neuronal connections in the mouse... be found on the Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...

  9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...taken up the plasmid. This can serve as an alternative to antibiotic selection. Plasmids of this type .... Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. We’ve listed...each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split... marking/selection: Expression constructs like plasmids often include GFP as a marker to help identify...techniques in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide... plasmids 101...

  10. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...love of plasmids! Each chose their favorite plasmid and replicated it. Can you guess each plasmid? Our ...obviously make for good costumes), science and our plasmid collection (what science has been hot this year... ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, ...Office Team was back up to their creative hijinks last year. They showed us their dedication to great customer...
Showing: 361 - 370 of 816 results