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Showing: 361 - 370 of 598 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...produced in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein ...

  3. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right...and the crRNA. The spacers of the crRNA can be customized as well, including concatenations into crRNA ...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...64 possible codons but only 20 amino acids and 3 stop codons to encode leaving 41 codons unaccounted for...preferences among organisms aren’t completely understood, but some possible reasons include: Metabolic...

  5. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

  6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... al is a PCR-based approach for generating a customizable comprehensive mutagenesis library that’s ready...sites in the same orientation as the gene or use custom gene synthesis or site directed mutagenesis to ...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...cloned by two separate labs (Bird et al., 1988; Huston et al., 1988). scFvs are the smallest unit of an.... https://doi.org/10.1371/journal.pone.0189964  Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotny...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...of such targets include transcription factors, histones, and DNA repair enzymes. Note that when using ...stepwise decrease in fluorescence intensity in a (histogram) flow plot (Figure 4b). Through this method, differences...

  10. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...
Showing: 361 - 370 of 598 results