We narrowed to 851 results for: CAL

...given the data provided by the depositing lab, we call these “quality control (QC) issues.” We then ask..., Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation...

...to visualize DNA, an editor targets DNA (via catalytically inactive dCas9) to modify chromatin. A fluorescent...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

... Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel recently took a different ...Strategy #2 (ProB): Create promoters using phylogenetically conserved sequences that are upstream of the...

...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing...

...into the genome, CRISPR may increase this rate. Specifically, portions of the AAV genome can integrate at...AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specificin vivoscreening platform. bioRxiv...

...interest, and the two helper plasmids, commonly called “triple plasmid transfection” (Figure 1).    Figure... ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration of viral particles...

...efforts make a precise edit or insertion via a chemical modifier. We used these categories to see what...function from 2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of ...

...sequence (gene). In a clean sample, there are typically fewer than 10 hits to a given sequence, and we...control is recommended but not yet required for clinical-grade materials, and is not commonly performed...

...directions. Drosophila are very easy to manipulate genetically and have limited genetic redundancy (meaning,...reference of various fly phenotypes, check out the Classical Genetics Simulator website. One interesting thing...

...contracts behind closed doors. Fig. 1: The mythical land of negotiations.    You negotiate everyday...are negotiating for a specific time slot on the confocal microscope. You’re trying to get better images...

...not as quantitative as other methods of PCR. Theoretically scientists should be able to determine the quantity...addition of a probe based fluorescent dye that intercalates with any dsDNA and the use of a fluorometer ...

...however, your cost per cloning reaction drops dramatically, as one tube of enzyme should be enough for ... guarantees, some cloning techniques do tip the scales in your favor. For starters, Golden Gate cloning...

...requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...results out there include: Google Groups - I've specifically set up a forum where NgAgo users can share protocols...

... to deaminate cytidine and used dRanCas13b (catalytically inactive Cas13) to target the cytosine deaminase... from these off-target edits helped to minimize local off-target editing by RESCUE. To address both C-...

...quickly along regions calling for abundant, charged tRNAs but will stall at regions calling for low abundance...

...but essentially we just needed to send a Google calendar invite over to the r/Science team with a description...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...

...guest blogger Samantha Young. Samantha Young is a Medical Writer with a PhD in reproductive biology and genetics...Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid...

...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ...PMCID: PMC225270. Higgins NP, Vologodskii AV. Topological Behavior of Plasmid DNA. Microbiol Spectr. 2015...

...the full plasmid sequence can be assembled or theoretical — it does not have to be entirely verified by... Lysine (D123K), lowers biosensor affinity for calcium". Please describe any tags or fusions (e.g. His...

... watching, and we hope you found the video radd-ically helpful!    More resources at Addgene Addgene'...