We narrowed to 771 results for: IRE;

1. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...plasmids, glycoproteins and other proteins that require post-translational modification may not be easily...

2. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...are many ways a user can input their query — by directly typing terms in a search bar, by ticking some ...

3. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

4. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...results we hoped for, but it did open up a new direction for the project. I was invited to participate ...

5. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

7. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ...the person you are talking to is really in your direct area.  Review the conference schedule and attendance...

8. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...is helpful to find peers that share a similar direction or problem. For example, a group of people who...

9. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's...

10. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

11. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...collaboration and growth opportunities, and gives your entire audience a chance to comfortably engage with you...

12. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in...

13. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...not so simple Just as our lab used jargon that required context to understand, I came to realize that ...

14. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...

16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...chemical modifications to the RNA to control the entire editing system with a simple LED light. Lighting...

17. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...it would take years to develop the mouse lines required for this purpose. Tang wondered if he could use...

18. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...Scientific Manuscripts. Any scientific manuscript will require numerous other references to scientific literature...

19. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...

20. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation in...