We narrowed to 751 results for: Nes;
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TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....website to verify plasmids or validating your new clones, try these tips to make the most of your time and...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m.... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m.... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....place to work, we do SO many fun things!!” Okay - honestly, maybe not everyone replies with quite so much... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in... -
Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... Common negative controls include knockout cell lines or tissues that do not express the protein. With... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m.... your gene to be in relation to the rest of the genes? And do you want it to be in the same (forward) ... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....below or browse Addgene’s collection of empty backbones. Our Most Popular Plasmids 101 Posts: Origin...