We narrowed to 781 results for: Por
-
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....research. But be aware that the fluorescent proteins reported in publications are only characterized under a...
-
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....understanding all of the players becomes ever more important to ensure that you choose the most appropriate... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m.... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA in a ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....while also capturing information about spatial and temporal organization. The design use to detect PKA activity... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m.... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m.... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...