We narrowed to 790 results for: Por

1. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...

2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

3. Plasmids 101: Blue-white Screening

   Type

   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

5. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

7. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

   Published

   June 16, 2022, 1:15 p.m.

   ... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

8. Antibodies 101: Secondary Antibodies

   Type

   Blog Post

   Published

   Dec. 21, 2021, 2:15 p.m.

   ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...

9. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective...

10. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take...

11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated...

12. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

13. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

15. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

16. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

17. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

18. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

19. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

20. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...