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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 848 results for: STI;

Showing: 721 - 740 of 848 results

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...far messier than biorender plots, quadrants can still help me as a reader if I'm struggling to see the...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  3. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... tag antibody produced by our partners at the Institute for Protein Innovation (IPI), part of a new collection... more highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged...

  5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ...Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate billions ...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved by...depending on the delivery method and cell type in question. CRISPR components can be delivered and expressed...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ... and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik ...homology-independent pathway as it involves the direct ligation of sticky or blunt ends and often introduces small insertions...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...1896) where he explores the concepts of animal testing and modifications. The premise of the novel involves...mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy to introduce point mutations...

  13. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... about antibody engineering technologies, the Institute of Protein Innovation (IPI) provides information...development methods. Despite recent advances, there is still always room to improve, and scientists will continue...

  14. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

  16. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types...they are grouped together because they contain a distinct Cas7-type gene. The exact mechanism of type IV...

  17. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses...

  18. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll ...

  19. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...competition, where you can get all your burning AAV questions answered.  Interested? You can learn more here...

  20. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...we were able to address these issues using our existing resources. You can read it, and other case studies...
Showing: 721 - 740 of 848 results