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Addgene

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We narrowed to 856 results for: TOR

Showing: 721 - 740 of 856 results

  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab in the ...supply house.  These large, cheap aliquots could be stored indefinitely at room temperature (thus also saving...

  2. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...) are echoed in the amount of interest in base editors, which alter genomic DNA without the need for cellular...editing rates across multiple systems. Read our introductory blog post, then check out this RNP protocol ...

  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...elements such as promoters and transcription terminators, the boundaries are less obvious. We found that...at the University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he ...

  4. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ... Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order ready-to-use antibodies...infrastructure, from our educational materials to our repository of open samples. We are, and have always been...

  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...Communication to diverse audiences Organization Mentorship Leadership Science writing competitions...Patent process Business Models Communication Regulatory knowledge Networking Blogging Writing...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid ...

  8. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ..., invite them to speak for your organization, mentoring group, or department. Even if it doesn’t work ...ask my benchmate to take my plates out of the incubator because I’d had a baby that night, all he said...

  10. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...presents equipment needs, such as shakers and incubators, and biosafety issues, such as how to dispose...activities fit your lesson plans. If you are an instructor at the college level, perhaps consider using ...

  11. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...PCR to generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, ...simple as mixing fragments together with linearized vector for seamless joining of multiple mutations spread...

  13. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development Plan Resources...

  15. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...

  16. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...interest is a low abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion...

  17. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC can...genome. REPLACR-mutagenesis starts out in familiar territory, but quickly diverges from previously discussed...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...
Showing: 721 - 740 of 856 results