We narrowed to 884 results for: ica

...Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions for defining the problem... training too many scientists and some propose radical changes to the way we view the graduate school ...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...at target sites. Read the paper in Nature Communications Find the Cas12b plasmids  Plant base editing...Microbiology Find the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics...

...conference, you could be honing your science communications skills and networking. And on social media,...one “safe” project that you know you can get a publication with and one “shoot for the moon” project that... the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator Networking “Many,...

...virtually. 6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider by Jennifer Tsang The...The nanodrop vs. spec debate dominates DNA quantification discussions, but what about the other methods... with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel To complement ...

...sequence in live cells. This technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing...

...2 trophies over the years, which is either an indication of the stiff competition or says something about...building activity that is easy to organize. We’ll dedicate a post to these competitions in the future, and...establish, with the goal of fostering better communication between labs. Scientists are often willing to...

...Annotation of genes in immune cells typically involves the creation of germline knockout mice, which ...Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in improving CD8+ T cell...screening of genes in the immune system. Nature Communications, 10(1), 1668. https://doi.org/10.1038/s41467...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... helps you reach your goals Listens well and communicates clearly and openly Gives honest feedback without...lab. If only every science trainee had such a dedicated person guiding the start of their career. “I want...

...important. It needs to express value, clearly communicate exactly what a site offers to their users, and....  With these three main values we set out to radically redesign our homepage to reflect this message....hug items closer and do so more animatedly. To communicate that sense of community, Blugene will regularly...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you have a new...

...control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs throughout...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...require a permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics...

...let curiosity guide your intellectual journey. Practical approaches One approach to achieve this goal ...chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars exposes you to...ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking...

...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

...There must be some way to use LEGOs, which are practically universally loved (except for those rare times...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...

...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

...reproducibility and an inability to improve upon these critical research tools. Addgene is excited to announce...in neuroscience research today, but there is a critical unmet need for an open-access, molecularly defined...such as plasmids and viral vectors. However, it typically takes a few years for a new project to reach a...

...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...

... that will bring about the next revolution in biological research. We’re incredibly happy to see that ... colonies to find a successful clone? Addgenie Jessica Welch explains how Blue-White screening could be...repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog...

...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success...