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We narrowed to 875 results for: ica

Showing: 721 - 740 of 875 results

  1. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9) derived from Streptococcus...methylation-silenced genes. Read the paper in Nature Communications Find Casilio-ME plasmids If you have a new...

  2. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...important. It needs to express value, clearly communicate exactly what a site offers to their users, and....  With these three main values we set out to radically redesign our homepage to reflect this message....hug items closer and do so more animatedly. To communicate that sense of community, Blugene will regularly...

  3. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...let curiosity guide your intellectual journey. Practical approaches One approach to achieve this goal ...chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars exposes you to...ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking...

  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs throughout...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...require a permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics...

  6. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...There must be some way to use LEGOs, which are practically universally loved (except for those rare times...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...

  7. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

  8. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  9. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...reproducibility and an inability to improve upon these critical research tools. Addgene is excited to announce...in neuroscience research today, but there is a critical unmet need for an open-access, molecularly defined...such as plasmids and viral vectors. However, it typically takes a few years for a new project to reach a...

  10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...

  11. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... that will bring about the next revolution in biological research. We’re incredibly happy to see that ... colonies to find a successful clone? Addgenie Jessica Welch explains how Blue-White screening could be...repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog...

  12. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success...

  13. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the coordinators...

  15. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

  17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...

  20. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ... specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...
Showing: 721 - 740 of 875 results