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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 845 results for: ins;

Showing: 721 - 740 of 845 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... for packaging, however, the isoforms of these proteins, as well as the viral LTRs differ. Therefore, ...Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of 96 retroviral...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    .... The advantages provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects.... PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a...

  4. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within... Eric J. Perkins...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... now specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx...optogenetic reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided...

  6. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...plasmid of interest, for detecting the presence of an insert or piece of DNA in a sample such as in colony PCR...into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification. Following...

  8. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via...

  10. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...light to distinguish one fluorophore from another. Instead of using a single (primary) detector to identify...narrow band, to discern one marker from another. Instead of compensation, which relies on user adjustment...

  13. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...information. Personally, I would much rather read the instructions than watch a how-to video. My dad, on the other...love it and others will avoid it like the plague. Instead of looking for The One Method to Rule Them All,...

  16. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...including casual request via email, in person, or instant messaging but we as scientists often prefer data...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...time to produce beta-lactamase prior to division, instead of immediately experiencing the effects of the ... capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11...

  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Eleven years later,  Addgene is a company of fifty instead of three, and we are sending hundreds of plasmid...yard this year, though she generously offered. Instead we picniced outside of our new office space on ...

  19. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...run and the long run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic...
Showing: 721 - 740 of 845 results