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  4. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

  19. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...
Showing: 721 - 740 of 776 results