We narrowed to 754 results for: tro

...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...

...little enrichment of DNA targeted by a negative control gRNA or predicted gRNA off-target sites. Long-range...

..., freeze them, and store all but one in liquid nitrogen. The frozen vials make up your master stock, while...

...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

...  Susanna Stroik...

... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

... Susanna Stroik...

... vectors that can be administered systemically (retro-orbitally, RO; or intracerebroventricularly, ICV...

...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...

...SHERLOCK had a few limitations when it was first introduced in 2017 so the lab made further modifications...

...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...