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We narrowed to 772 results for: INA

Showing: 371 - 380 of 772 results

  1. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... precious in less resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...this was hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered...

  2. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...kill bacteria, or inhibit their growth. Although originally sourced from natural products, many common antibiotics... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...

  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...unsuccessful. Nothing more joyful that getting to the final step and the transformed bacteria refuse to grow...(Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S...matrices, not so much. Maybe a calcium-independent proteinase site could be used for plasmid version 2.0? Instruments...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

  5. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

  6. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...mobilized to figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...scientists think that they are unable to tick these final two boxes, but I knew there would be a way around...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series...SMART.” SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea ... open communication Feel valued by giving back Final tips for success Make mentoring meetings a priority...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  10. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...heterogeneous and/or mixed clones present in the original sample. At seqWell, we have worked with Addgene...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...
Showing: 371 - 380 of 772 results