...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...to once again reflect on our achievements in the last year and share the exciting things to come in the...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

...scFV antibody, which is expressed from a separate plasmid. This system amplifies the intensity of the fluorescent...SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter...The high SunTag signal allowed them to cut their laser power by over 80% and still obtain a higher signal-to-noise...imaging deep inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked single molecules...basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it’s clear that the...Fluorescent Protein Guide Page Find Luciferase Plasmids ...

... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

...articles (3/12/2014). With so many options for CRISPR plasmid tools and numerous experimental design decisions...genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR genome editing experiment...with expression, though it is possible for the plasmid to still express without the G. Because the G is...marker to select transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459...related to the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools...by a variety of labs and optimized for specific plasmids Links to different software to help you identify...

...indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype...dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses...serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8  pAAV-hSyn-mCherry...channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA...55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-...

...Greek for “self-eating”) is a process by which cytoplasmic material, including organelles, are targeted ... untargeted Rosella remains in the neutral pH cytoplasm where it emits red and green fluorescence. Following...clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused with a mitochondria-targeting...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe... autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana... Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent protein guide: Biosensors...

...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586...