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We narrowed to 816 results for: LAS

Showing: 371 - 380 of 816 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... of the Plasmodium falciparum genome. To put this last point into perspective, the Plasmodium falciparum... over 90% in their work editing the genome of Plasmodium falciparum (the parasite that causes malaria)...with the post-design process such as primer and plasmid design. Examples:  “multiplex design”, “multi-method... same organism could be used as a proxy for this last approach. Top tool for first timers: You need to...also the only tool I have come across to offer classes of parameters based on the type of CRISPR experiment..."Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Communications Biology Find the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE...paper in mSphere Find the dCas9 and dCas9-Mxi1 plasmids If you have a new CRISPR tool you’ve recently...

  3. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to bacteria... library or an especially low concentration of plasmid), you will benefit from purchasing competent cells...survivalTM, and Stbl3TM. How to transform your plasmid DNA If you decided to use commercial competent ...negative control (plate your cells without adding plasmid) to make sure you don’t have contamination.  ... Also conduct a positive control (test a plasmid that transforms well in other competent cells) to ensure...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...insect cells, they are most often expressed from a plasmid in bacteria (Ahmed et al., 2012). One challenge... to form. When expressed in the non-oxidizing cytoplasm of bacteria, scFvs accumulate in insoluble inclusion...expression in bacteria:  targeting to the oxidizing periplasm of bacteria to allow for proper disulfide bond...are redox mutants so they have a more oxidizing cytoplasm. expression in the presence of molecular chaperones...chaperones that help scFvs fold properly in the cytoplasm (Sandomenico et al., 2020). conversion to a cysteine...cysteine free format that’s easier to express in the cytoplasm of E. coli, although this is not possible for ...Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies for neuroscience research...

  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang...RESCUE. Read the paper in Science Find RESCUE plasmids If you have a new CRISPR tool you’ve recently...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...Menassa's labs made the accidental discovery that laser treatment can photoconvert GFP from green to RED...Photoconverted GFP spreads through the cytosol via cytoplasmic strands. Image from Sattarzadeh et al., Sci Rep... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place of the epitope...Jürg Bähler have deposited a large collection of plasmids for genome modification in yeast developed by ...Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome modifications in ...Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional ...

  8. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...to once again reflect on our achievements in the last year and share the exciting things to come in the...factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single deposit in...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

  9. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

  10. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...scFV antibody, which is expressed from a separate plasmid. This system amplifies the intensity of the fluorescent...SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter...The high SunTag signal allowed them to cut their laser power by over 80% and still obtain a higher signal-to-noise...imaging deep inside the cell, in the nucleus and cytoplasm. Again, they found that SunTag marked single molecules...basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it’s clear that the...Fluorescent Protein Guide Page Find Luciferase Plasmids ...
Showing: 371 - 380 of 816 results