We narrowed to 771 results for: INA

1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...downstream of the transcription start site will terminate transcription of most of the ncRNA (Figure 1D)...

2. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...strength of bands from different rounds of detection. Finally, stripping and reprobing works best with western...

3. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   Published

   March 7, 2024, 2:39 p.m.

   ...are not in your favor. Thus, there is a need to eliminate or reduce the PAM requirement to allow flexibility...

4. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...state only by simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased...

5. Antibodies 101: Choosing the Right Isotype

   Type

   Blog Post

   Published

   April 6, 2023, 3 p.m.

   ...the background staining but they do not always eliminate the issue (Lu 1998). Consequently, if you find...

6. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ...other ways to make your poster more accessible. Final thoughts Though we’ve just presented some standard...

7. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

   Type

   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...proof-of-principle, the team used Nanoblades to disrupt the tyrosinase (Tyr) gene in mouse zygotes, which results in...

8. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...microscopy and computer imaging processing. The combination of these methods provides unique opportunities...

9. Early Career Researcher Toolbox: Finding Relevant Papers

   Type

   Blog Post

   Published

   Jan. 9, 2020, 1:45 p.m.

   ...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications...

10. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ... Keira Havens grew up in Hawaii where she was fascinated by flowers, bugs, and the ocean. After receiving...

11. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...possibility that this might just be an artifact and eliminate sources of interference, but the result persisted...

12. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ... you should be able to run your experiment and finalize your compensation values later. This can be helpful...

13. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...

14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...of only the broadest-targeting variants. For the final round of evolution, they used a no-G PAM library...

15. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate with...

16. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...contain two dimerizing FPs can exist in an open inactive state, a closed active state, and an artificially...

17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...transduction or injection as either protein, RNA, or DNA. Finally, once you have identified the best expression system...

18. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...GFP also emits yellow light). Note that some combinations of FPs can cause an effect called FRET (fluorescence...

19. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...polls and surveys. A mix of closed (multiple choice/binary) and open questions is helpful to ensure acquisition...

20. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ...colleagues at NYU and visiting scientists, such as seminar speakers. They made an every effort to interact...