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We narrowed to 847 results for: PLE

Showing: 741 - 760 of 847 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...specific epitope that your antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le...be the antibody Mary Poppins!   All of the examples of new versions of Myc1-9E10 mentioned above basically...

  3. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed...transcribed gRNA are combined to form a Cas9-gRNA complex and delivered to cells using cationic lipids. Transient...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered in his laboratory. Learn...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...range of issues related to AAV vectors, many from people trying to answer the exact same question I was:...Sharing data and community concerns AAV vectors are complex biological reagents; many factors, like storage...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...producer cells where there is E1 and E3 gene complementation. In target cells that are infected by the vector...Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... own proteins, making the purification process simpler. Due to its high yields, E. coli is often used ... the purity of your antibody prep. If you have multiple contaminating bands or a few strong ones, consider...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...PAM or sgRNA sequences Cas9 likely goes through multiple rounds of cleavage at the target site until either...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

  10. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry(orange..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...

  11. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...incompatible? Plasmid incompatibility occurs when multiple plasmids within one cell have the same replicon...Schumacher, 2012). This concept is a little more complex than simply competing for replication control factors...

  12. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...done by a single enzyme; however, eukaryotes have muliple polymerases which are each responsible for a specific...speaking, promoters in bacteria are less diverse and complex, having fewer parts than those in eukaryotic cells...

  13. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility, selective...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...have various disadvantages and limitations – for example, microtubule-destabilizing drugs are impossible... embryo (Aljiboury et al., 2023). It’s a great example of how optogenetics allows you to disrupt and study...

  15. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...BL21 strain and derivatives are the most common examples of the E. coli B strain. Common E. coli strains...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...modifications. CRISPR base editing persists through multiple cell divisions, indicating that this method produces... Multiple Authors...

  17. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  18. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ... you don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to ...

  19. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...
Showing: 741 - 760 of 847 results