We narrowed to 790 results for: Por

1. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

2. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

3. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

5. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

6. Experimenting in an Open Source lab: from CRISPR to Cats

   Type

   Blog Post

   Published

   July 23, 2015, 2:30 p.m.

   ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

7. Common Injection Routes in Mice

   Type

   Blog Post

   Published

   Jan. 22, 2025, 9:42 p.m.

   ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

8. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

9. Antibodies 101: Flow Compensation

   Type

   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

10. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...

11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody...

12. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

14. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

15. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...

17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...production process? These questions are especially important when producing multiple vectors in parallel because...

19. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...community, will soon expand to include several other important pathways. We invite feedback and contributions...

20. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...