We narrowed to 781 results for: Por
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TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....community, will soon expand to include several other important pathways. We invite feedback and contributions...
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Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....Researchers from a variety of institutions recently reported their inability to recapitulate the results of... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....change your compensation values during the analysis portion of your experiment. So, as long as you are able... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m.... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....passage are too young and should not be used for important transfection experiments right after they’ve been... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m.... Signaling and Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m.... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....& Kappes, J. C. (1997). Functional RT and IN incorporated into HIV-1 particles independently of the Gag... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...