We narrowed to 842 results for: RING

1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

   Type

   Blog Post

   Published

   July 18, 2017, 12:41 p.m.

   ... cry genes from the soil bacterium Bacillus thuringiensis and analysing the insecticidal potential of ...

2. Fluorescent Proteins 101: Fluorescent Biosensors

   Type

   Blog Post

   Published

   May 18, 2017, 2:30 p.m.

   ...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...

3. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ... Mary Gearing...

4. FastCloning: A Newer, Simpler Cloning Technique

   Type

   Blog Post

   Published

   Nov. 1, 2016, 2:30 p.m.

   ...plasmid to confirm no mutations were introduced during PCR or any subsequent steps. Using high fidelity...

5. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...regular, informal feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening...

6. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...

7. Antibodies 101: Epitope Tags

   Type

   Blog Post

   Published

   May 11, 2023, 1:15 p.m.

   ...ideal with Myc, as low pH conditions are required during the purification, which may affect protein function...

8. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   Published

   Oct. 10, 2019, 12:41 p.m.

   ...stable frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672...

9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...changes favored blue-shifting more than others. During this process, they identified mWasabi, a bright...

10. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists would be able to ...

11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...This instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR ...

12. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

13. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction...

14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2015, 2:30 p.m.

    ...edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content...

15. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ... Mary Gearing...

16. "Build Your Plasmid": The Game - Play to Teach

    Type

    Blog Post

    Published

    July 17, 2018, 12:23 p.m.

    ...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease...

17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    Published

    March 30, 2023, 1:15 p.m.

    ...differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced with the TF pooled library...

18. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    Published

    Sept. 3, 2019, 1:15 p.m.

    ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam...

19. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ... track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased ...

20. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius...