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We narrowed to 856 results for: TOR

Showing: 741 - 760 of 856 results

  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA sequences for your next....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

  7. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...., Langhorst, B. W., Bilotti, K., Pryor, J. M., Cantor, E. J., Canton, B., Knight, T. F., Thomas C. Evans...J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...? In this post we will review some of the main factors that affect isotype choice. Target and application...when choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety...

  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

  10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...cells with Nanoblades Primary cells are often refractory to gene delivery methods, but the researchers...

  11. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...the experiments performed by our thousands of depositors. That means their original articles are your ...searches scientific articles, patents, and even some doctoral dissertations. Streamline the question asking...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...a method  As with all experiments, a number of factors will determine which approach is right for your...number measurement of the low-affinity Fc gamma receptor genes FCGR3A and FCGR3B. PloS one, 10(1), e0116791...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

  14. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...include the paper, any supplemental data, software repositories, or other information needed by someone to critically...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...In mathematics, I think this is represented by factorials. In environmental science and ecology, this can... sentences, make it flow, and start telling the story. Like any editing process, you will need time away...

  17. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...exclusive. In the first, the replication initiation factor RepA binds to the iteron to initiation replication... than simply competing for replication control factors and tends to be more of a concern with low copy...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson...selection of codon optimized genes, so check out our inventory before synthesizing a gene yourself.  Caveats ...

  19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen species, ...plasmids that liberate the targeting region from the vector. Alternatively, PCR-generated or commercially synthesized...
Showing: 741 - 760 of 856 results