We narrowed to 867 results for: TOR

1. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted...meant that the posters could be viewed publicly and stored in one place if the participants used the conference...

2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   Published

   Nov. 20, 2018, 8:17 p.m.

   ...persistent and exist online indefinitely. PIDs historically referred to objects that exist online, but this...to ensure that materials available through our repository are verified and identifiable to save researchers...

3. Management for Scientists: Delegating is Key

   Type

   Blog Post

   Published

   Feb. 20, 2015, 8:30 p.m.

   ...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development Plan Resources...

4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ...confirm the presence of an insert in a particular vector by excising the insert from the backbone. To do...will need to know both the approximate size of the vector backbone as well as the predicted size of the insert...

5. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...interest is a low abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion...

6. Clear and Accessible Writing

   Type

   Blog Post

   Published

   Feb. 15, 2024, 2:15 p.m.

   ...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...

7. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...for your experiments, there are three important factors to consider: site accessibility, specificity, and...less likely to elicit a strong immune response, factors that may make it especially suitable for in vivo...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...is then digested and ligated into an appropriate vector. Gibson Assembly, GeneArt Cloning, and SLIC can...genome. REPLACR-mutagenesis starts out in familiar territory, but quickly diverges from previously discussed...

10. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...

11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA sequences for your next....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

12. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...

13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, ...of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid...

14. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August...proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals...

15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

16. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

17. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...., Langhorst, B. W., Bilotti, K., Pryor, J. M., Cantor, E. J., Canton, B., Knight, T. F., Thomas C. Evans...J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot...

18. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...with Beckman Coulter and that certainly played a factor in our decision to go with this machine, but we...Management Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources...

19. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...? In this post we will review some of the main factors that affect isotype choice. Target and application...when choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to the ...cells with Nanoblades Primary cells are often refractory to gene delivery methods, but the researchers...