We narrowed to 763 results for: ire;
-
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...
-
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m.... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....And, many wanted to learn more, even those not directly involved in the project. Once we gauged interest... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m.... to help the DNA enter the cells. Some samples require a reduced antibiotic concentration or need to be... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH 0.8 Affinity and Purification... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....pre-set timers for incubation steps, and save the entire process so you’ll have a record of your work. This... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....that charming fixer-upper house you have always admired or volunteer to lead a charitable cause. While... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....the production of a cell surface glycan that’s required for ricin uptake. The ricin screen did fail to... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m.... addition to the neuronal networks involved in directly controlling muscle activity and coordinating movement... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m..... The supporting data and community engagement required to do this drove Addgene to develop new systems... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....lines of showing that the checkout takes the user directly to the shipping step, and no longer takes the ... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....Your mental health and success in science will directly depend on your science mentor. One scientist who... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....gene editing process. See below for details. The entire process from start to finish took about 1 month...