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  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...passage are too young and should not be used for important transfection experiments right after they’ve been...

  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

  11. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...you have used blastn to determine the reliable portion of a Sanger sequencing result and noted any potential...

  12. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou...

  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...26 of a 30 nt target sequence. For the editing portion of the protein, they examined ADAR1 and ADAR2, ...
Showing: 741 - 760 of 776 results