We narrowed to 772 results for: INA
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TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...
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FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....community-editable resource for storage and dissemination of information about FPs. FPbase.org FPbase...data to existing proteins when missing, or edit inaccuracies as they find them (anyone can register with ...community-editable database is free from the risk of inaccurate data submissions, so FPbase includes a versioning... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m....DNA preps with multiple samples without cross contamination. We currently use the CosMCPrep system for this...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m....these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m...., cyan, or magenta) depending on the hairpin combination. A gRNA containing all three hairpins produces...generates blue, green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA...increase stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m.... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work. We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m.... sign up here. New toolkit for N or C terminal tagging of proteins for degradation using AID ...created a new toolkit of plasmids for the N- or C- terminal tagging of proteins with mAID and other tags (...SpCas9-NG with the activation-induced cytidine deaminase Target-AID-NG, which mediated a C to T conversion... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed... 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci..., L. Liang, L. Luo, The Q system: a repressible binary system for transgene expression, lineage tracing... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....to break membranes of different organisms. And finally, we demonstrated that R bodies can be “tuned” with...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...University of British Columbia. Originally on track for a research career, she transitioned into a communications...