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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: LAS

Showing: 381 - 390 of 816 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your Plasmid Background...appropriately sized product, purify and sequence the plasmid to confirm no mutations were introduced during ...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find...Browse Our Special Collections for Plasmids from Your Field ... plasmid cloning...

  2. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Resources & Plasmids Plan Your CRISPR Experiment Synthetic Biology Resources & Plasmids  ...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated...poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited and distributed...expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

  4. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...In fact, an entire classification scheme, known as the Baltimore replication classes, has been developed...replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016 Viruses with DNA genomes... an RNA or dsDNA intermediary, depending on the class of virus. RNA genomes can be transcribed into mRNA... of RNA or DNA intermediaries, depending on the class of virus. From one, come many After a virion infects...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors...

  5. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...indicators Plasmid Serotype Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST Chemogenetics Plasmid Serotype...dimmer than iGABASnFR, and accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses...serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5, AAV8  pAAV-hSyn-mCherry...channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA...55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...Greek for “self-eating”) is a process by which cytoplasmic material, including organelles, are targeted ... untargeted Rosella remains in the neutral pH cytoplasm where it emits red and green fluorescence. Following...clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused with a mitochondria-targeting...decrease in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe... autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana... Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent protein guide: Biosensors...

  8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and... contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

  9. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...studying four of those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome...involving the primary cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1...there. This will help us acquire a lot of those plasmids so that we can try many different things in parallel..., will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations...experienced by AHC patients. Waardenburg Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol...mutations. More specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr... patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation. Plant Cell 24:1478–1493...Steinmetz LM, Drewes G, Huber W (2019) Biological plasticity rescues target activity in CRISPR knock outs....
Showing: 381 - 390 of 816 results