We narrowed to 783 results for: INA

...series!  This post was originally written by Didem Goz Ayturk and updated by Ina Ersing in August 2023 ... your final stocks only contain rAAV. To minimize contamination, use appropriate decontamination procedures...40% PEG solution to each 100 mL of supernatant (final concentration of 8%). If, however, you’ve collected...digital PCR (ddPCR) (Lock et al., 2014), or determination of the median tissue culture infective dose ...primers targeting the viral genome or inverted terminal repeats (ITR). You should always have a high-quality...adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy Methods, ...Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular...

...Brian O’Neill and Ina Ersing for contributing their expertise! This post was originally published in January...administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can...Carboxypeptidase D (CPD), also called AAVR25   AAV9 Terminal N-linked galactose3 AAV receptor (AAVR)2 Laminin...found to transduce skeletal muscle, cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated...tropism, including liver, muscle, lung, CNS, kidney, retinal, and pancreatic cells (Ahuja et al., 2025; Issa...cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006...transduce liver, skeletal muscle, pancreatic, and retinal cells (Issa et al., 2023). AAV11 AAV11 is a relatively...

...) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR...system. The FLP recombinase recognizes FRT sites and initiates site directed gene recombination between those...channel under the control of Cre recombinase are used in combination with mouse lines that express Cre...regulated recombinase - keeping Cre out until needed But what can be done when the recombination should ...concentrations in combination with low concentrations of vectors expressing the required recombinase. The high...under the control of Flp recombinase together with inducible Cre recombinase (CreERT2), visualizes individual...Tamoxifen injection induces Cre-ERT2 recombinase, leading to recombination of the loxP sites and thereby to...

...components into a destination vector in a predefined order. Finally, the destination vectors are used ...positional groups. Eight final destination vectors, consisting of all four final RVD modules in each of ...promoter, N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant...Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis construct...light-activated receptor tyrosine kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface...determined by competing recombination events at the genetic level. Upon Cre/loxP recombination, each transgene...:1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also...

...models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors...Causes of recombination and leaky expression While leaky expression and spontaneous recombination is a known...sets of homologous recombination sites, they hypothesized that spontaneous recombination between these sites...even begins. This spontaneous recombination persists even in recombinase deficient E. coli and under conditions...vectors are much more prone to recombination and can exhibit recombination levels as high as 1-3%. In light...the recombination rate The first step in optimizing DIO/FLEx vectors is to know the recombination rate...system is off-target or spurious expression of recombinase-dependent AAV vectors. In this article, we will...

...against random recombinants.   Design your targeting construct. For recombination to occur in a ... for recombination, the negative selection marker selects against improper, random recombination into ...random recombination may incorporate the negative selection marker into the genome. This final product...After recombination has occurred, you'll first remove the resistance marker using Cre recombinase. Since...are 3 loxP sites, recombination can occur in multiple ways. The desired recombination event will remove...strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting...yeast, mice, or Drosophila through homologous recombination. The concept is simple: deliver a template with...

...44632, the contaminant we mixed into the original AAV sample at 5%. Detecting 5% contamination is a good... Our recombination software looks for and quantifies the presence of recombinants in recombinase-dependent...sequences. Recombinase-dependent sequences may be subject to recombinase-independent recombination during ...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...prep we suspect is contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid ...This recombination produces a minor pre-recombined population that does not require the recombinase for...viral vectors and estimate that recombinase-independent recombination occurs in 0.1-0.6% of AAV particles...

...human kinases remains challenging. Small molecule kinase inhibitors usually target soluble kinase domains...thereby rapidly create desired allele combinations and combinatorial genomic libraries. MAGE has allowed...pORTMAGE) expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the... HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions. Kim, et al. Mol... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...Human kinase domain constructs for automated bacterial expression The misregulation of human kinases has...recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed...

... manipulation. Cre recombinase, originally named because it “causes recombination” (although later referred...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination at the...alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites...separate recombination events? One potential limitation of using loxP sites is the inability to tightly...specific recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system... 1. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. Sternberg, N. and.... 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Sauer...

...deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids first, ...be used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic capsids... X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed...promoters and enhancers, miRNA target sites, and recombination target sequences, denoted by triangles] facilitate.... In cases where it’s critical to minimize or eliminate off-target expression, it may be better to choose...capsids in our repository are deposited by the Gradinaru lab. You can visit our Caltech Systemic Capsids...