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...background, which means that each mouse is genetically identical and all mice of the strain are homozygous...create a limited but genetically diverse gene pool. Outbred strains are strategically bred to maintain maximum...using the same nuclease may not result in genetically identical mice. Thus, scientists must genotype each...organism used to understand mammalian traits and genetically engineered mouse models provide researchers with...useful and adaptable tools to perform basic and preclinical research. For scientists new to using mouse models...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...) National Institutes of Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting...

... Predoctoral Fellow at University of Rochester Medical Center. During development, complex genetic programs...genetic underpinnings of these processes has been complicated by the lack of precise genetic tools for modulating...central nervous system (CNS). To address this technological gap, a trio of recent papers describe the development...recent advancements and offer commentary on their application to neuroscience research. What is CRISPR-based...with different untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used ...

...some tips for performing this purification.  Check out the AAV Purification protocol page for more detailed...! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This...This video goes over AAV vector purification using an iodixanol column gradient. We'll show the loading... tissue culture as it is commonly called, is a critical tool for many scientists. Mammalian cells provide...provide scientists with the means to study biological processes on the cellular level instead of having ...

... the types of viruses used as biological tools.   Viruses as biological tools In nature, viruses infect...enough to ensure the virus can replicate and spread. Instead, viruses replicate by hijacking the host’s system...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward...Capacity (~4.5kb From basic science to clinical applications, viruses are true bench to bedside tools... why viruses are such wonderful scientific and medical tools. As scientists, we can manipulate what this...effectively infect a wide range of cell types that are typically ‘difficult to transfect’, such as primary cells...straightforward across applications, while electroporation of a human, for example, isn’t a reasonable option. Since...

... the Communicating Science Conference series for graduate students. I believe that communication is the...skill in science communication over time.  Like most skills, great science communication comes with practice...able to communicate with their colleagues and peers. Scientists also need to be able to communicate to expert...careers in science communication The strength of story telling Science communication: 9 strategies to get... world is the value that you can succeed in communicating. Even the most rigorous, insightful, and novel...scientist's career is mediated by some kind of communication to another person.  In an interview setting,...you do to ensure that your skills in science communication will be a positive asset throughout your career...

...repair Adapter Ligation Clean up/Size selection Amplification Clean up  I found this kit easy to use because...DNA we started the process with, we got great amplification of our sample libraries. NEB also gave us a ...mostly used to make new libraries as opposed to replicating them like we do at Addgene), going to the workshop...

...placing the calcium indicator GCaMP6f in the effector position, as well as to optogenetically control the depolarization...researchers to label just a fraction of a single genetically-defined subset of cells. By labeling fewer cells...While transgenic animals are commonly used to specifically manipulate a cell type of interest in an organism...Drosophila require heat-shocking the system or using chemical inducers of gene expression (del Valle Rodríguez...cells are not labeled. PhiC31, a recombinase. It specifically integrates an attP site into an attB site. In...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe. Image ...Image from Isaacman-Beck et al., 2019. Applications for SPARC SPARC technology is versatile and can be ...

...about Julie Cicalese  Ms. Cicalese served as the Chief Human Resources Officer for Harvard Medical School ...excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human...Human Resource Officer for Harvard Medical School. The addition of Cicalese to the Board of Directors brings...Human Resources Association, Association of American Medical Colleges and the Society for Human Resource...When I was working in Human Resources at Harvard Medical School, Joanna was my colleague. She mentioned ... science but my prior HR experience at Harvard Medical School means I understand the needs of scientists...where I foster dogs and help screen dog adoption applications. My most recent foster dog was with us for two...

...science communication are as varied as the field itself. Among other things, science communication comprises...suspect that science communication might be for you, don’t wait. Start communicating now. These 9 strategies...museums, zoos or botanical gardens. You can also work or volunteer for a science communication organisation...project. Science communication opens doors Getting involved in science communication has been the most...career researcher looking to break into science communication? Everybody goes about this in their own way....hashtag #scicomm and start following science communication societies like @BritSciAssoc, @AAAS, @Stempra.... Use Twitter to promote your other science communication activities, e.g. tweet it when you publish a...

...protein for your application. Selecting the best fluorescent protein for a specific application can seem like...Here, we will explain how practical brightness is determined and why practical brightness is a better criterion...criterion than theoretical brightness for selecting the brightest fluorescent protein. Theoretical brightness...higher the number, the higher the theoretical brightness. The theoretical brightness often appears in tables...proteins based on their practical brightness in your experimental set up. The practical brightness takes all...same reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...which is a key determinant of the practical brightness. Therefore, practical brightness provides a better ...