...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...constructs in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...improved genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors...viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue voltage... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...

... successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene...including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked by recombination...transformed with the plasmid backbone (with or without your inserted DNA) and further screening will help you...example, Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy...allows the bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this... finding more and more ways to creatively perform selections in plasmid cloning. Additional resources...purify your gene of interest, get it into your plasmid backbone, and zap the mixture of DNA into cells...

...Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2...see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...to provide them to their students. My desire to create a set of low-cost, easy-to-use educational biology...fragrances that they can smell, or enzymes that can create hydrogels that they can touch. With BioBits, we...

...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...repair by HDR facilitates in-frame insertion of tag-encoding sequences at the intended target site. With the...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...single-stranded donor DNA (ssODN) that contains the tag-encoding sequence and short homology arms (60-70 bp) is...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...targeting the gene of interest) and ssODN (tag-encoding donor DNA) using our TAG-IN bioinformatics tool.A...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope... spike protein, scientists encode the spike protein on the envelope plasmid. Image from Crawford et al...with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of...

...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can be...vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be...Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding...expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium...

... heavy chain proteins as well as the genes that encode those proteins are designated by the lower-case...co-expressing IgM and IgD isotypes antibodies which are encoded by the heavy-chain constant genes μ and ẟ. These...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing the...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for imaging of...for quantitative imaging, Ming Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach...was cloned into bacterial expression vectors to create a library. Bacteria were transformed, and then ...for in vivo studies. Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be...

...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...expression. This is a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors... technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are expanding the inventory...sensor (see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV...reduced. This variant has an approximately 12-fold increase in brightness after ~100 action potentials. This...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...CRISPRi system, the Laub lab repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...

...Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is comprised...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ... key advantage of KTRs is that they are easy to create and simple to multiplex. Problems with FRET kinase...

... cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40-70 kDa), which is half...regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain....sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...diagnostic toolkit, called DETECTR, which has been created by the authors. This kit uses Cas12 and Cas13 to...

...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM,...Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent Protein Should I...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...

...selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection ...percentage of surviving cells may be the result of plasmid integration via recombination during transfection...production of virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could... prior to their transduction has been shown to increase AAV transduction efficiency by approximately 15...efficiency depends on viral titer Both of these can be increased by concentrating a viral stock. Different titering... virus-cell contact A variety of reagents can increase transduction efficiency. These reagents typically...reagent that is commonly used and has been shown to increase transduction efficiency by 10-fold (Davis et al...

...are transfected with plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate...downstream effects.  Bryan Roth’s lab created a collection of plasmids called TRUPATH to interrogate 14 of...their mechanism. These plasmids are available as a collection, facilitating screening across the transducerome...But with approximately 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream...TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different experimental approaches...Addgene.org Browse the catalog of fluorescent protein plasmids Find biosensors for your experiments ...resulting in decreased BRET2 signal. With increased concentration of drug, the BRET2 signal decreases. ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...melanogaster. Get the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...different neurotransmitters. Using genetically encoded fluorescent proteins coupled with Fibronectin intrabodies...targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...YGOI in a pattern that allows the function of the encoded protein to be assayed (e.g. pPD95_86 and pPD57_...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...

...spatial control. Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular...reporter gene in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model...approach. These researchers used a genetically encoded photocaging technique to insert a light-removable.... presented data showing that this genetically encoded, photocaged Cas9 system could silence endogenous...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400-fold increase after 30 hr. The system...(CIB1) proteins. The goal of both groups was to create a system that would use light to turn on and off...