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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 435 results for: Trac

Showing: 91 - 120 of 435 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...could then be used to examine both inter- and intrachromosomal dynamics, and how they change in response ...turned their attention to mapping pairs of intrachromosomal loci. The technique successfully resolved ... this work represents the first mapping of intrachromosomal loci, a major benchmark in characterizing ...

  2. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...have: working to deadlines, writing up clear and attractive reports, being good at presentations and of course...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True...

  3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...not possible to elicit an immediate response or track the exact time of activation. Receptor activity ... Mouse studies, however, require a permanent intracranial implant to be surgically installed to allow ...receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit; inhibits...in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in mind that some E. coli strains...

  5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...same frequency, we reasoned that, following DNA extraction, the [+] and [-] strands might naturally associate...restriction enzyme based methods, we showed that the DNA extract is a heterogeneous mix of single-stranded and double-stranded...species. While not all of the molecules in the DNA extract associate with complementary strands, enough double-stranded...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ..., the DNA of interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

  7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant”. His proposal, however...1960s when scientists began to study other extrachromosomal particles, particularly Resistance or R-factors...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...

  8. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...setting writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android  ...  This app lets you track habits, like writing. It’s basically the equivalent of crossing off days on ...

  9. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ... mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying a reagent...making it difficult or impossible for researchers to track down that resource. A more long-term solution is...IDs in published work allows readers to quickly track down the specific plasmids used. RRIDs and resolvable...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:ATP ratio. Most of...sensors developed for it. Calcium triggers muscle contraction, and calcium channels are activated for neuronal...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...instead of by another method? Viral delivery is attractive due to the ease of transduction in difficult-...human transcription factors, barcoded libraries to trace cell lineage, and so much more! Libraries allow ...as delivery mechanisms for gene therapy. The tractability of AAV, low immunogenicity, and cell-specific...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...RNA cargo into their targets makes them attractive and tractable tools for genome engineering. Scientists... into an organism. This feature makes them an attractive tool for human subject delivery, as immunogenicity...

  13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ... on size is ultracentrifugation. Sedimentation equilibrium analytical ultracentrifugation yields an affinity...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

  14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...raised or hours tracked, 3% of postdocs were at institutions focused only on tracking hours, and 22% of...postdocs at the University of California, who had a contract - negotiated by their union - securing higher ...

  15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...the lab observed abnormal cell migration and contraction. The lab hypothesized that this is abnormally...identify whether any rescue cell migration and contraction defects. The lab also plans to use CRISPR plasmids...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has...

  16. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly used to deliver...neurons and mouse brain slices collected following intracranial injection of an AAV expressing a ChRger. In ...nervous system which eliminates the need for intracranial injections. When packaged in PHP.eB, ChRgers...

  17. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...of using social networking to start, build, and track professional relationships. Here are my seven best... for example). This is also a great way to keep track of how you met the connection, as these notes are...my paper resume). It’s also a great way to keep track of past accomplishments. Follow all the rules of...

  18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original version containing...photoactivatable fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity at the plasma membrane...intensity at different time points, the authors tracked protein activity while also capturing information...

  19. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...BioID or Split-BioID, cells are lysed, protein extracted, and labeled protein interactors purified via ...speed of labeling. BioID requires 6-24 hours for tetracycline induced expression of the BirA* fusion protein...Split-BioID, Schopp et al allowed 24 hours for tetracycline induced expression and biotinylation of interacting...

  20. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...localization in cells, or even single-particle tracking of the bound complex. There are so many FPs available...TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells... can even be bright enough for single-molecule tracking in live cells.  Controls  When designing experiments...

  21. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...protocols.io.brgcm3sw     New system developed to track cell-cell interactions By Jennifer Tsang GFP-based...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

  22. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...particles (Figure 1). Briefly, packaged DNA is extracted from purified, DNase-treated AAV and submitted...with AAV-68544 to validate our method. We then extracted the DNA and processed the mixed sample in our ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org...

  23. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Cellular activities like action potentials, muscle contractions, and the control of voltage gated ion channels...insufficient level. The heartbeat – a muscle contraction – is directly related to the electrical impulse...and emit signal from. This specific example is attractive due to the large number of sheer molecules and...

  24. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...protein of interest. Modified proteins can then be extracted from lysed cells through the use of protein tags...forming inclusion bodies that are difficult to extract without harsh reagents and patience. Plant expression... of complex post-translational modifications. Extraction and purification of recombinant proteins from...

  25. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...ultrafugation Overview: Iodixanol gradient ultracentrifugation uses a gradient of different concentrations...during AAV production is overlaid. Following ultracentrifugation, the AAV-containing 40% fraction is collected...day. Refer to the AAV Iodixanol Gradient Ultracentrifugation Protocol for more details. Pro tips Watch...

  26. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... method (Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA ...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional ...the targeted region (Sanson et al., 2018) . This tracrRNA was combined with the sgRNA expression vector ...

  27. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...kb), and they remain popular for targeting and tracing cells in the brain (Figure 1). 2nd generation lentiviral...been used for complex applications like lineage tracing in heterogeneous tumors. Lentiviral vectors are...In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola D, Riecken K,...

  28. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

  29. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

  30. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...certain number of days and tracking average transit time for each courier we contracted with so we can always...Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial ...
Showing: 91 - 120 of 435 results