960 results

Review of ways to prepare yourself for training in a lab, optimizing training time, and positively contributing to bench mentor-mentee relationships.

Sept. 26, 2023, 1:15 p.m.

Using anti-mouse antibodies in a mouse model can result in high background signal. Here are three ways to overcome this challenge.

Sept. 21, 2023, 1:15 p.m.

Review of the most common types of affinity tags and there optimal usages.

Sept. 19, 2023, 1 p.m.

Interested in learning more about synthetic biology but not sure where to start? The Codon Guide to Synthetic Biology may be the resource you're looking for!

Sept. 14, 2023, 1:15 p.m.

A list of free online molecular biology tools for plasmid mapping, DNA and protein sequence analysis, primer design and more.

Sept. 12, 2023, 1:15 p.m.

Addgene is pleased to announce the release of the 4th edition of ours Plasmids 101 eBook!

Sept. 5, 2023, 1:15 p.m.

A review of a new brain to gut neural fiber technology from the Anikeeva and Bohorquez labs.

Aug. 31, 2023, 1 p.m.

Review of what a visiting scientist is, why researchers choose to visit other labs, and how to prepare for these types of appointments.

Aug. 22, 2023, 1 p.m.

Curious about what a virus is, and how it differs from a viral vector? Check out our Viral Vectors 101 blog that explains it all!

Aug. 18, 2023, 4:15 p.m.

Learn about a variety of AAV encoded calcium biosensors available from Addgene.

Aug. 10, 2023, 1 p.m.

If you're designing a flow panel with a large number of markers, it might be time to look into spectral cytometry instead of conventional flow.

Aug. 3, 2023, 1:15 p.m.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

Learn about the structure and life cycle of retroviruses to help understand how to use viral vectors tools in the lab.

July 27, 2023, 1:15 p.m.

Description of the components of the 4 most common viruses - AAV, adenovirus, lentivirus, and gamma-retrovirus.

July 25, 2023, 1 p.m.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, provides tips on getting a faculty positions & fulfilling your academic career goals.

July 20, 2023, 1:15 p.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

Find the basics about chemogenetics: what are chemogenetics, why use them, and how to build your chemogenetic system using receptors and ligands.

July 11, 2023, 1:15 p.m.

A review of the types of engineered biosensors and how viruses can be used to deliver them.

July 6, 2023, 1 p.m.

The Addgene marketing co-op shares her experience working at Addgene for the past five months.

June 29, 2023, 7 p.m.

We describe the different classes of optogenetic tools available and what levels of control they give you in your experimental setup.

June 27, 2023, 1:15 p.m.

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

June 22, 2023, 1:15 p.m.

Expansion microscopy allows for light microscope imaging of fixed tissues while overcoming the 250 nm resolution limit.

June 20, 2023, 1:15 p.m.

Curious about how you can expand the number and type of amino acids available in your experiment? Genetic code expansion can help!

June 15, 2023, 1:15 p.m.

The Institute for Protein Innovation and Addgene are partnering to share IPI's high-quality, well-validated recombinant antibodies.

June 13, 2023, 1:15 p.m.

Learn about the IBEX Knowledge-Base, which allows researchers to share validation data on imaging reagents for IBEX imaging.

June 8, 2023, 1:15 p.m.

An overview of viruses used in biological research. Information related to history, applications, and limitations of the four most common viruses.

June 6, 2023, 1:15 p.m.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

June 1, 2023, 1:15 p.m.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

May 30, 2023, 1:15 p.m.

Seqera recently published a case study on Addgene's use of Nextflow to build a better bioinformatics platform.

May 25, 2023, 1:15 p.m.

If you need to image more than one protein in your sample, multiplex immunofluorescence is an important skill to master!

May 23, 2023, 1:15 p.m.

Ever wonder if the Fc region did more than just provide structure and isotype specificity? It actually has a range of immune functions we cover in this post!

May 18, 2023, 1:15 p.m.

A review of the advantages of epitope tags and commonly used tags and their antibodies.

May 11, 2023, 1:15 p.m.

Highlights of CRISPR impact in the drug discovery pipeline prior to clinical advancement.

May 4, 2023, 1 p.m.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

May 2, 2023, 1 p.m.

Learn how you can use retrograde AAV for neuronal tracing.

April 27, 2023, 1:15 p.m.

Right variable region, but wrong constant? Chimeric antibodies may be the answer!

April 25, 2023, 1:15 p.m.

If you present data to others, you have a valuable transferable skill: public speaking!

April 20, 2023, 4 p.m.

A brief history of HEK cells and their unique applications and uses.

April 18, 2023, 1:15 p.m.

An overview of viral applications spanning from basic research science to clinical therapies.

April 13, 2023, 1:15 p.m.

Want to show how often your plasmids are requested? Or where they're being shipped to? The depositor-developed Addgene Request dashboard can help!

April 11, 2023, 4 p.m.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

April 6, 2023, 3 p.m.

A review of K. phaffii as a model organism and its relevant applications for biotech to basic research.

April 4, 2023, 1 p.m.

Overview of the MORF library collection and accompanying ATLAS deposited at Addgene

March 30, 2023, 1:15 p.m.

Watch carefully as we walk you through how to run a Western blot in this protocol video!

March 28, 2023, 1:15 p.m.

How much time does it take to clone a plasmid and how much does it cost? We do some back of the envelope math to try and figure it out!

March 23, 2023, 1:15 p.m.

Viral research basics and their use as biological tools. Learn about retrovirus, lentivirus, adenovirus, and AAV.

March 21, 2023, 1:15 p.m.

A review of statistical testing basics and the most common parametric comparison tests.

March 7, 2023, 2:15 p.m.

Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

March 2, 2023, 2:15 p.m.

The science community has gotten pretty good at sharing open software. But sharing open hardware brings its own unique challenges and solutions.

Feb. 28, 2023, 2:15 p.m.

In her first blog post, Addgene Executive Director Chonnettia Jones reflects on highlights of 2022 and shares exciting plans for 2023.

Feb. 23, 2023, 2:15 p.m.